Signalling

Part:BBa_K3425106:Design

Designed by: Rubén Crespo Blanco   Group: iGEM20_UofUppsala   (2020-10-08)


pCadBA testing construct (pSB3K3)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2729
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2729
    Illegal NheI site found at 1384
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2735
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2729
    Illegal XhoI site found at 177
    Illegal XhoI site found at 1020
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2729
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2729
    Illegal XbaI site found at 2744
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 1470
    Illegal AgeI site found at 1793
    Illegal AgeI site found at 3467
    Illegal AgeI site found at 3579
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2498


Design Notes

Copy number seems to affect greatly the level of expression attainable by this part. Also, it's important to not have excess expression of BBa_K3425100 to prevent leaky expression. Also, remember that BBa_K3425101 alone does not have an RBS!


Source

Same as BBa_K3425101

References